Isolation of MSCs From Bone Marrow

Mesenchymal stem cells isolated from bone marrow of rat/mice.
Take femur and tibia and flush them with medium.
Incubated cells at 37 degree with 5 % carbon dioxide.
when MSCs attach to plastic surface change medium.(this medium contain hematopoietic cells as they are non adherent so they remains in the medium).
After every 3 days medium will be change.
when cells become confluent subculture them and use for further experiments.